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. 2016 May 9;6:25628. doi: 10.1038/srep25628

Figure 7. SIRT6 is required for the protective effect of Nutlin-3 on Aβ42-induced DNA damage.

Figure 7

HT22 cells were transfected with SIRT6-specific siRNA. Twenty-four hours after transfection, cells were treated as indicated for an additional 24 h. (A) Western blot analysis for SIRT6, p53 and γH2AX levels. β-actin and histone H3 were used as loading control. (B) Quantification of γH2AX level is shown in (A). Statistical significance was tested by one-way ANOVA followed by a Tukey’s multiple comparison test (n = 5, *P < 0.05 versus vehicle, #P < 0.05 versus Aβ42-treated groups). All the gels were run under same experimental conditions. Full-length images are presented in the supplementary information.