Fig. 3.

Skap1−/− T-cells show alterations in anti-CD3 induced talin cleavage. (A) Naïve CD3⁺ T-cells from Skap1+/+ or Skap1−/− mice were activating with cross-linking anti-hamster antibodies alone (0) or with anti-CD3 cross-linked with anti-hamster antibodies for either 10 min (10) or 30 min (30). The cytoplasmic and membrane fractions were isolated as indicated and immunoprecipitated with anti-talin. A negative control sample immunoprecipitated with IgG1 antibodies was included (lane 7). The immunoblots were probed with anti-talin to detect the full length (∼235 kDa) or rod-domain of talin (∼180 kDa). (B) The relative densitometric reading of the full length or rod-domain from lanes 1–6 for the membrane fraction were measured using ImageJ.