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. 2016 Apr;172:40–46. doi: 10.1016/j.imlet.2016.02.004

Fig. 4.

Fig. 4

Non-cleavable talin L432G rescued the conjugation defect of SKOT1CTLs. CTLs were transfected with either empty EGFP (EGFP), talin-EGFP or talin-L432G-EGFP vectors. (A) In the presence of DC-OVA, SKOT1CTLs transfected with EGFP ([SKOT1 vector EGFP] mean ± SD, 610.0 ± 33.7 s) showed significant decrease in contact time as compared with OT1 transfected with EGFP. (B) Displacement of SKOT1 CTLs transfected with EGFP were significantly lower in contrast to OT1 CTLs transfected with EGFP in the presence of DC-OVA (p < 0.05). The displacement of SKOT1 CTLs transfected with talin-EGFP was comparable to those transfected with EGFP alone (p > 0.05). Transfection of Talin-L432G into SKOT1 cells restored displacement to levels seen with OT1 cell transfected with EGFP alone in presence of DC-OVA.