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1.3% denaturing agarose gel used to resolve Okazaki fragments from wild-type and cac1 mutant straints. Fragments were end-labeled using Klenow enzyme and α-[32P]dCTP. Labeled samples were loaded with 6X denaturing loading buffer and bromophenol blue and resolved for 5h at 80V. The chromatin repeat is easily visualized in the wild-type sample, but is missing in the CAF-1 deficient strain, as expected.
