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. 2015 Dec 1;1:15034. doi: 10.1038/celldisc.2015.34

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Copyright © 2015 SIBS, CAS

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CDC73 silencing leads to genome instability. (a and b) Average number of γH2AX and P-ATM foci in U2OS cells depleted for CDC73 is significantly increased compared with control (*P=0.027 and ***P=0.001, respectively). (c) Comet Assay with siRNA-transfected U2OS cells. CDC73 depletion leads to significantly increased DNA damage as analyzed by the average tail moment (***P=0.004). (d) Normal S-phase distribution in CDC73-depleted cells based on PCNA staining. (e) CDC73 knockdown cells do not undergo cell cycle arrest and show a normal rate of BrdU incorporation as analyzed by FACS and propidium iodine counterstain. (f) CDC73 silencing does not lead to apoptosis measured by the amount of cleaved PARP1 on western blot. For all experiments n⩾2. Average and s.d. are plotted. P-values are calculated with Student’s t-test and scale bars represent 50 μm.