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. 2015 Nov 17;1:15033. doi: 10.1038/celldisc.2015.33

Figure 3.

Figure 3

Disruption of ER network integrity compromises endosome velocity. (a, b) GFP-HDEL, GFP-RTNLB3, YFP-RabF2a and YFP-RabA1g were transiently expressed in tobacco leaf epidermal cells. GFP-HDEL reveals a typical ER network with tubules and cisternae; however, expression of GFP-RTNLB3 causes deformation of the ER network. Scale bars, 5 μm. (c, d) Streaming velocity measurements of 165 endosome organelles labeled by YFP-RabF2a (c) and 218 endosomes labeled by YFP-RabA1g (d) in tobacco epidermal cells in the absence (solid green lines) or in the presence of overexpressed GFP-RTNLB3 (solid red lines) were calculated from kymographs generated in ImageJ (v1.49 h). Endosome movement is expressed as histograms and probability density function (PDF) of the velocity distributions, which were fitted (blue and magenta dashed lines) using distribution tool (Matlab). Inset in c and d display the average velocity of the relative endosomes. Statistical analysis was performed using Student’s t-test (***P<0.0001). ER, endoplasmic reticulum.