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. 2015 Nov 17;1:15033. doi: 10.1038/celldisc.2015.33

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Copyright © 2015 SIBS, CAS

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Endocytosis is delayed in rhd3. (a, c) Time course (5 min, 15 min, 1 h) to follow uptake of the endocytic tracer FM4-64 in Col-0 and rhd3 seedlings. (b, d) Fluorescence signal intensities represented as heat map facilitates visualization of levels of FM4-64 uptake in the two genetic backgrounds. (e) The graph shows a quantification of the FM4-64 signals in the root epidermis (fluorescence signal, after 5 min of FM4-64 treatment, was measured at plasma membrane and cytosol as indicated by the white dashed rectangles in a). Statistical analysis was performed using Student’s t-test (***P<0.0001). (f) The graph represents the percentage of FM4-64 internalized looking at the cytosolic area. (g) BFA bodies observed in the maximal projection of confocal images of 6-day-old Arabidopsis seedlings (Col-0 and rhd3 root epidermal cells) pulsed with FM4-64 marker and treated with 100 μM BFA for 1 h. BFA, brefeldin A.