Figure 1.

ANG expression levels in the SQCLC cancerous tissues versus adjacent normal tissues, and verification of ANG overexpression in Ad-ANG SK-MES-1s. A, RT-PCR showed that ANG mRNA levels were significantly higher in cancerous tissues than in adjacent normal tissues in SQCLC patients (n = 3 experiments for all tests). * P < 0.05 versus adjacent normal. B, Local expressions of ANG and HMGA2 protein were upregulated in cancerous tissues. C, The transfection efficiency of adenovirus vectors in SK-MES-1s, which was assessed under fluorescence microscope, reached ≤ 70%. D, mRNA level of ANG was significantly enhanced in Ad-ANG SK-MES-1s (n = 3 for each cell group). * P < 0.05 versus control. Ad-GFP served as control. E, Protein levels of ANG and HMGA2 were significantly upregulated in Ad-ANG SK-MES-1s. All experiments were carried out in triplicate.