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. 2016 Apr 28;7(7):862–871. doi: 10.7150/jca.14440

Figure 2.

Figure 2

ANG is an important regulator of proliferation, migration, invasion, and apoptosis of SK-MES-1s. A, A higher optical density at 450 nm (OD 450) in the CCK8 assay was observed in Ad-ANG SK-MES-1s at 24, 48, and 72 hours (n = 3 for each cell group). * P < 0.05 versus control. B and E, Results of the migration chamber assay showed that migration capability was significantly higher in the Ad-ANG SK-MES-1s. Quantified data were presented as the number of migrated cells per high-power field (HPF) (n = 3 for each cell group). * P < 0.05 versus control. C and F, Transwell invasion results showed that invasion capability was significantly higher in Ad-ANG SK-MES-1s. Quantified data were presented as the number of invasion cells per HPF (n = 3 for each cell group). * P < 0.05 versus control. D and G, Cells were stained with Annexin V-FITC and propidium iodide (PI). Flow cytometric contour plots showed the percentage of the stained cells. Histogram showed the percentage of Annexin V+/PI- and Annexin V+/PI+ cells. Ad-ANG cells showed a lower apoptotic rate (n = 3 each cell group). * P < 0.05 versus control. All experiments were carried out in triplicate.