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. 2016 Apr 28;7(7):862–871. doi: 10.7150/jca.14440

Figure 6.

Figure 6

HMGA2 is a mediator of ANG in regulating proliferation, migration, and invasion of SK-MES-1s. A and B, Successful knockdown of HMGA2 in Ad-ANG SK-MES-1s. Ad-ANG SK-MES-1s transfected with shScramble served as negative control (n = 3 for each cell group). * P < 0.05 versus control. C, CCK8 assay after HMGA2 knockdown in Ad-ANG SK-MES-1s. A lower OD 450 value was observed in Ad-ANG/shHMGA2 SK-MES-1s at 24, 48, and 72 hours (n = 3 for each cell group). * P < 0.05 versus control. D and F, Ad-ANG/shHMGA2 cells showed a lower migration ability, as compared to Ad-ANG/shScramble SK-MES-1s. Quantified data were presented as the number of migrated cells per HPF (n = 3 for each cell group). * P < 0.05 versus control. E and G, Transwell invasion results showed that invasion capability was significantly lower in Ad-ANG/shHMGA2 SK-MES-1s. Quantified data were presented as the number of invasion cells per HPF (n = 3 for each cell group). * P < 0.05 versus control. H, The apoptotic rates difference in Ad-ANG/shHMGA2 and Ad-ANG/shScramble SK-MES-1s showed no statistical significance (n = 3 for each cell group). ▲ P > 0.05 versus control. All experiments were carried out in triplicate.