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. Author manuscript; available in PMC: 2016 May 9.
Published in final edited form as: Methods Enzymol. 2015 Jun 2;560:91–116. doi: 10.1016/bs.mie.2015.04.012

Table 2.

Kinetic parameters of EcTrmD and MjTrm5.

Parameter EcTrmD MjTrm5 Assays References
Km (tRNA), µM 3.1 ± 0.1 0.70 ± 0.03 steady-state (Elkins et al., 2003)
(Masuda et al., 2013)
kcat, s−1 0.09 ± 0.01 0.017 ± 0.002 steady-state (Christian and Hou, 2007)
0.020 ± 0.007 pre-steady-state (Christian et al., 2010b)
Kd (tRNA), µM 0.44 ± 0.04 1.4 ± 0.1 single turnover (Christian et al., 2010b)
Kd (AdoMet), µM 0.8 ± 0.1 single turnover (Christian et al., 2010b)
Kd (m1G37-tRNA), µM 0.2 ± 0.1 pre-steady-state (Christian et al., 2010b)
Kd (AdoHcy), µM 0.020 ± 0.005 pre-steady-state (Christian et al., 2010b)
kchem, s−1 0.09 ± 0.01 0.12 ± 0.03 pre-steady-state (Christian et al., 2010b)
0.12 ± 0.01 single turnover (Christian et al., 2010b)