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. Author manuscript; available in PMC: 2016 May 9.
Published in final edited form as: Methods Enzymol. 2015 Jun 2;560:91–116. doi: 10.1016/bs.mie.2015.04.012
Reaction components Volume to add (µL) Final concentration (µM)
tRNA/TE 10.0 0.1–25
Heat-cool (HC buffer) 2.0
5× buffer 5.0 1.0×
3H-AdoMet (310 µM, 2,650 dpm/pmol) 3.0 37.2
EcTrmD or MjTrm5 (25 nM) 5.0 0.005
Total 25.0