Reaction components | Volume to add (µL) | Final concentration (µM) |
---|---|---|
tRNA/TE | 10.0 | 0.1–25 |
Heat-cool (HC buffer) | 2.0 | |
5× buffer | 5.0 | 1.0× |
3H-AdoMet (310 µM, 2,650 dpm/pmol) | 3.0 | 37.2 |
EcTrmD or MjTrm5 (25 nM) | 5.0 | 0.005 |
Total | 25.0 |
Reaction components | Volume to add (µL) | Final concentration (µM) |
---|---|---|
tRNA/TE | 10.0 | 0.1–25 |
Heat-cool (HC buffer) | 2.0 | |
5× buffer | 5.0 | 1.0× |
3H-AdoMet (310 µM, 2,650 dpm/pmol) | 3.0 | 37.2 |
EcTrmD or MjTrm5 (25 nM) | 5.0 | 0.005 |
Total | 25.0 |