| Syringe #2 | Volume | 2× Concentration | 1× Concentration |
|---|---|---|---|
| EcTrmD or MjTrm5 (400 µM) | 1.5 µL | 2.0 µM | 1.0 µM |
| ddH2O | 238.5 µL | ||
| 5× buffer | 60.0 µL | 1× | |
| Total | 300.0 µL |
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