| Syringe 1 | Volume | 2× Concentration | 1× Concentration |
|---|---|---|---|
| G37-tRNA transcript (100 µM) | 1.5 µL | 0.5 µM | 0.25 µM |
| ddH2O | 209.5 µL | ||
| 5× buffer | 60.0 µL | 1× | |
| Working stock of 3H-AdoMet | 29.0 µL | 30.0 µM | 15.0 µM |
| Total | 300.0 µL |
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