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. 2015 Aug 18;1:15021. doi: 10.1038/celldisc.2015.21

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Copyright © 2015 SIBS, CAS

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3AA and XYT472B do not inhibit secretase activities. (a) ELISA-based γ-secretase activity assay. HEK293T membrane fractions were incubated with chemicals and APP-TM peptides for 2 h before the ELISA analysis of the processed products. (b) Cellular APP processing was unaffected by 3AA or XYT472B treatment. HEK293/APPswe cells were incubated with chemicals for 4 h. Cell lysates were prepared and subjected to western blotting analysis of C99, C83 and APPswe. Culture media were collected for sAPPβ analysis. (c and d) Cellular γ-secretase activity was monitored and found unaltered in (c) C99-GVP and (d) NotchΔE-GVP reporter assays. Two hours after transfection, HEK293T cells were treated with chemicals. Luciferase reporter activities were measured 24 h after transfection.