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. 2015 Oct 27;1:15030. doi: 10.1038/celldisc.2015.30

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Copyright © 2015 SIBS, CAS

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Human melanoma cells (A375, Skmel28 and 1205Lu) (a and b) or melanoma cells freshly isolated from patients tumors (d) were transfected with 50 nm of either siRNA control (SiCt) or siRNA CD271 (SiCD271) for 48 h or at indicated time. At the end of experiment, viable cells were counted using trypan blue dye exclusion method and proteins were extracted and then subjected to western blot using indicated antibodies. ERK serves as a loading control. (c) A375 melanoma cells were transfected either with SiCt, SiCD271, Sicasapse-3 or combination of both. Forty-eight hours after transfection, proteins were extracted and CD271, PARP, caspase-3 and ERK were evaluated by western blot. The data showed the mean±s.d. of three independent experiments versus control (*P⩽0.05; **P⩽0.01; ***P⩽0.001).