Figure 5.

Melanoma cell lines sensitive (S) or resistant (R) to vemurafenib (a) or cells isolated from patients biopsies before (S) and after acquired resistance (R) (d) were stained with anti-CD271 antibody. The percentage of CD271-positive cells was analyzed by flow cytometry and CD271 protein expression level was determined by western blot. Sensitive (S) or resistant (R) melanoma cells were transfected either with SiCt or SiCD271. Twenty-four hours after transfection, cells were exposed or not to vemurafenib (Vem) 2 μm for 48 h. Viable cells were counted using trypan blue dye exclusion method (b and e). Proteins were extracted and CD271, PARP and ERK were evaluated by western blot (c and f). ERK was used as a loading control. The data showed the mean±s.d. versus control of three independent experiments (*P⩽0.05; **P⩽0.01; ***P⩽0.001).