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. 2015 Jul 14;1:15016. doi: 10.1038/celldisc.2015.16

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Copyright © 2015 SIBS, CAS

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Amer1 (2–700) with critical A1/A2/A4 interface residues mutated was not able to recruit APC to the plasma membrane. EGFP-tagged FL Amer1 or WT/point mutants of Amer1 (2–700) as well as FL APC were transiently transfected into MCF7 cells, followed by immunofluorescence analysis. APC was detected using the antibody Ali. Arrows indicate the localization of Amer1 and APC. (a, aʹ) FL Amer1 recruited APC to the plasma membrane. (b, bʹ) WT Amer1 (2–700) also carried APC to the plasma membrane. (c, cʹ) The A1/A2 sites double mutant D330K/D503K of Amer1 (2–700) still translocated APC to the plasma membrane. (d, dʹ) The A1/A2/A4 sites triple mutant D330K/D503K/E370K of Amer1 (2–700) failed to recruit APC to the plasma membrane.