Figure 3.

AC133-IR700-mediated PIT delays tumor initiation by NCH421k GBM-SCs in an s.c. mouse model. (A) NIR-FMT and BLI of mice bearing s.c. inoculated NCH421k GBM-CSs before and after PIT. NCH421k cells were pre-incubated with the AC133-IR700 conjugate overnight before cell implantation. NIR light (100 J/cm²) was applied to the implantation site in the left, but not in the right, flank immediately after implantation. Representative images are shown. (B) Inhibition of tumor initiation assessed by tumor volume measurements (n = 4; ****p < 0.0001; two-way repeated measures ANOVA). (C) Representative image of a mouse bearing two NCH421k tumors 50 days after inoculation. (D) Tumor growth assessed by BLI correlates with the volumetric measurements (n = 4). (E) Excised tumors 60 days after cell implantation.