Skip to main content
. 2016 Apr 21;6(6):896–914. doi: 10.7150/thno.14915

Figure 1.

Figure 1

Rhabdomyolysis promotes renal damage by oxidative stress and tubular apoptosis. C57BI/6 mice (males, 12 weeks old) were injected with saline or 10 ml/kg 50% glycerol in each thigh caudal muscle. Creatinine (A), BUN (B) and urinary myoglobin (C) were elevated from day 1 to 3, decreasing at day 7. (D) Representative images showing hematoxylin and eosin staining, confocal TUNEL images, and immunhistochemical 4-HNE, HO-1 and Ferritin images of mouse kidneys, scale bar 50 µM. (E) Quantitative TUNEL positive cells analysis in mice with rhabdomyolysis. Expression of tubular injury biomarkers Kim-1 (F) and Ngal (G), as determined by real time RT-PCR, in kidneys from mice with rhabdomyolysis. HO-1 expression, as determined by western-blot (H) and RT-PCR (I) was elevated 24h after glycerol-injection; whereas ferritin presence was increased from day 1 to day 7, as determined by Western-blot (J). Mice (n=5) per day-group. Results are expressed as mean ± SE. * p<0.05 vs non-treated mice.