Figure 3.

Rhabdomyolysis induces renal macrophages recruitment in a patient with rhabdomyolysis-induced AKI. (A) Creatinine (Cr) and creatine kinase (CK) serum levels indicated severe rhabdomyolysis-AKI in a 73-year-old patient that required dialysis for 14 days. Renal biopsy was performed on day 24. (B) Representative inmunohistochemistry showing hematoxylin and eosin staining (upper panel), total infiltrating macrophages (CD68, middle panel) and CD163-macrophages (CD163, lower panel) in the renal biopsy from with rhabdomyolysis-associated AKI, scale bar 50 µM. Healthy renal tissue was used as control. Histological analysis reported increased interstitial macrophage infiltration and the presence of acute tubular damage (white arrows), with tubules showing dilatation and flattening of the epithelial cells and containing brown granular material and protein casts (grey arrows), heme-iron deposits (yellow arrow). The rectangle shows the region of interest for which high-magnification images are shown in the right panels. (C) Representative confocal microscopy images showing co-localization of CD68 (green) and CD163 (red) in macrophages within kidney of the patient with rhabdomyolysis, scale bar 10 µM. Nuclei were stained with DAPI (blue). TL: tubular lumen. (D) Representative confocal microscopy images (left panel) and semiquantitative assessment (right panel), showing co-localization of CD206 (green), CD163L1 (green) and CD163 (red) in macrophages within kidney of the patient with rhabdomyolysis, scale bar 10 µM. Nuclei were stained with DAPI (blue).