Skip to main content
. 2016 Apr 21;6(6):896–914. doi: 10.7150/thno.14915

Figure 7.

Figure 7

Rhabdomyolysis induces renal fibrosis. Representative images showing kidney collagen content by Sirius red (A) and semiquantitative assessment (B) in mice with rhabdomyolysis, scale bar 100 µM. Expression of fibronectin (FN), type I collagen (Col I), alpha smooth muscle actin (α-SMA) and the pro-fibrotic mediator transforming growth factor (TGF-β), as determined by RT-PCR (C) and western-blot (D). Protein expression values were corrected by loading control (Tubulin). Mice (n=5) per group. * p<0.05 vs non-treated mice. In other hand, to determine whether macrophages were involved in rhabdomyolysis-associated fibrosis, we analyzed the expression of fibronectin (FN), type I collagen (Col I) platelet derived growth factor (PDGF), connective tissue growth factor (CTGF) and transforming growth factor type beta (TGF-β ) at mRNA level (F, H) or protein secretion (G) in supernatants from myoglobin (1mg/mL)-stimulated murine peritoneal macrophages. Murine tubular epithelial cell line (MCT) were also treated with myoglobin (1mg/mL) to determine the expression (I) and release (J) of extracellular matrix proteins (Col I and FN). Results are expressed as mean±SE of at least three independent experiments. * p<0.05 as compared with non-treated cells.