Figure 1.

Ack interacts with Yki and promotes tissue growth. (a) Ack interacts with Yki. S2 cells expressing Myc-Yki or blank vector were immunoprecipitated and probed with indicated antibodies. Experiment was repeated three times; and representative blots are shown. (b) A schematic of Hippo signaling pathway. (c) Ack promotes the transcriptional activity of the Yki–Sd complex in a kinase-dependent manner. S2 cells were transfected with the indicated constructs; and the cell lysates were subjected to dual luciferase assay. Quantitative data are expressed as mean±s.d. (triplicate wells). ***P<0.001. (d) Ack overexpression promotes tissue growth in vivo. Wild-type male wing and wings expressing MS1096-Gal4 with UAS-V5-Ack or UAS-V5-Ack-KD were shown. Ack and Ack-KD were inserted at the same attp site to ensure equal expression level. Overexpression of Ack induces wing overgrowth compared with control, whereas overexpression of Ack-KD suppresses wing growth. Quantification of wing area of each group is expressed as mean±s.d. (N=10). ***P<0.001. Scale bar is 500 μm. (e–j) Ack promotes eye growth and enhances Yki activity. Wild-type adult eye (e) or eyes expressing the indicated constructs (f–j) were shown. Dashed line indicated normal eye size. Scale bar is 300 μm. Experiments were repeated; and representative eyes are shown. (k) Yki RNAi suppresses Ack-induced wing growth. Wild-type male wings and wings expressing indicated constructs were shown. Quantification of wing area of each group is expressed as mean±s.d. (N=10). ***P<0.001. n.s., not significant. Scale bar is 500 μm.