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. 2016 Feb 23;2:15047. doi: 10.1038/celldisc.2015.47

Figure 2.

Figure 2

Ack overexpression upregulates Hippo pathway targets. (a–i′) Ack overexpression upregulates Yki target genes. Wild-type wing discs (a, a′, d, d′ and g, g′) or wing discs expressing UAS-V5-Ack (b, b′, e, e′ and h, h′) or UAS-V5-Ack-KD (c, c′, f, f′ and i, i′) by hh-Gal4 were immunostained to show the expression of Ci and Hippo pathway markers, including Ex-lacZ (a–c′), diap1-GFP4.3 (d–f′) and bantam sensor (BS) GFP. Note that, in the P compartment, Ack overexpression upregulates the expression of Ex-lacZ and diap1-GFP and downregulates BS GFP, while Ack-KD overexpression cannot (g–i′). Arrows indicated the P compartment. White dashed lines indicated the A-P compartment boundary based on Ci staining. Scale bar is 100 μm. (j–k′′′) Drosophila wing discs expressing UAS-V5-Ack (j–j′′′) or UAS-V5-Ack-KD (k–k′′′) by hh-Gal4 were immunostained with Yki and V5 antibody. Compared with the A compartment, in the P compartment, Ack overexpression promotes Yki nuclear localization whereas Ack-KD overexpression cannot. l–l′′′ and m–m′′′ are magnified pictures of the dashed line indicated P compartment area in j–j′′′ and k–k′′′, respectively. DAPI was stained to show nuclei. White dashed line indicated the A-P compartment boundary. Scale bar is 20 μm.