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. 2016 Mar 22;2:16006. doi: 10.1038/celldisc.2016.6

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Copyright © 2016 SIBS, CAS

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Overexpression of Tai promotes tissue overgrowth induced by Yki and activates the expression of Hippo pathway target genes. (a–d′) Side (a–d) and dorsal (a′–d′) views of wild-type adult eye (a, a′), eyes expressing UAS-Tai (b, b′), UAS-Yki (c, c′) and UAS-Yki+UAS-Tai (d, d′) with GMR-Gal4. Scale bars, 100 μm. (e–h) BrdU incorporation in wild-type eye discs (e), eye discs expressing UAS-Tai (f), UAS-Yki (g), or UAS-Yki+UAS-Tai (h) with GMR-Gal4. Arrowheads indicate the position of second mitotic wave. Scale bars, 50 μm. (i–l′) Drosophila discs containing flip-out clones expressing UAS-Tai by act>CD2>Gal4 (AG4) were immunostained to show the expression of diap1-lacZ (i, i′), ex-lacZ (ex-Z) (j, j′) or bantam-GFP (BS) (k–l′). Clones expressing UAS-Tai were marked by the lack of CD2 staining and indicated by arrowheads. Scale bars, 50 μm.