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. 2016 Mar 22;2:16006. doi: 10.1038/celldisc.2016.6

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Copyright © 2016 SIBS, CAS

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Characterization of different domains of Tai. (a) Schematic diagram of the N- and C- truncations of Tai protein. (b) TaiC sufficiently enhances the activity of Sd-Yki to trigger 3xSd2-luc reporter gene whereas TaiN does not. S2 cells were transfected with indicated constructs and luciferase reporter gene, followed by dual luciferase assay. The data were quantified using an unpaired t-test. The results represented the mean± s.e.m. ***P<0.001, **P<0.01, n.s., no significant difference (n=3). Note that the activity of TaiC is even higher than that of Tai full length. (c) Schematic diagram of the different domains of Tai protein. (d) CoIP of Myc-tagged TaiP2, TaiP3, Tai-P5 and Flag-Yki. S2 cells were transfected with indicated constructs, followed by immunoprecipitation and western blot analysis with indicated antibodies. (e) Tai-P5 acts synergistically with Sd-Yki to activate 3xSd2-luc reporter gene. Tai acts synergistically with Sd-Yki to activate 3xSd2-luc reporter gene. S2 cells were transfected with indicated constructs and luciferase reporter gene, followed by dual luciferase assay. The data were quantified using an unpaired t-test. The results represented the mean± s.e.m. ***P<0.001, **P<0.01, *P<0.1, n.s., no significant difference (n=3). (f) Schematic diagram of the different variants of Tai protein C-terminus. (g) TaiC-ΔP3 enhances the transcriptional activity of Sd-Yki while TaiC-ΔP5 does not. The data were quantified using an unpaired t-test. The results represented the mean±s.e.m. ***P<0.001 (n=3), ***P<0.001, **P<0.01, *P<0.1, n.s., no significant difference (n=3).