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. 2016 Mar 15;2:15050. doi: 10.1038/celldisc.2015.50

Figure 5.

Figure 5

TRIB2 attenuates the leukemia initiation potential of NOTCH1. (a) Kaplan–Meier survival analysis of the lethally irradiated recipient (CD45.1+) mice transplanted with 5-FU-enriched WT or Trib2−/− BM cells (donor: CD45.2+) transduced with either MigR1 control or ICN1 transgene. The number (n) of mice analyzed was from three independent experiments. Engraftment of transduced donor cells (CD45.2+GFP+) (b) and development of T-ALL leukemia (CD4+CD8+) (c) in moribund mice were verified by flow cytometry analysis. The values in the outlined areas (c: bottom row) are frequency of leukemic cells in the BM of moribund mice. Leukemic burden was assessed by the spleen to body weight ratio (d) and WBC counts (e) of moribund mice. (f) Leukemic infiltration was assessed by measurement of the frequency of leukemic cells in various organs of moribund mice. (g) Activation of MAPK signaling in T-ALL was determined by western blotting for p-Erk, total Erk, p-p38, total p38, p-JNK, JNK1 and β-actin signals in the leukemic BM (n=4 per group). Signals shown were developed from triplicate immunoblots. For a, Log-rank test was used to compare the survival curves. **P=0.0053, all quantified data are presented as mean and s.e.m. GFP, green fluorescent protein; WBC, white blood cell.