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. 2016 Jan 5;2:15039. doi: 10.1038/celldisc.2015.39

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Copyright © 2016 SIBS, CAS

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hNit1 interacts with β-catenin. (a) FLAG-tagged β-catenin forms a complex with myc₆-tagged hNit1 in co-immunoprecipitation experiments with anti-FLAG M2 antibody. (b) Endogenous hNit1/β-catenin complexes can be co-immunoprecipitated from lysates of HeLa and HEK-293 cells with monoclonal anti-Nit1 (1C3) antibody. (c) PLAs using monoclonal anti-Nit1 (1C3) and polyclonal anti-β-catenin (M14M) antibodies confirm intracellular interaction of hNit1 with β-catenin. shNit1, MCF-7 clone 6 stably transfected with shNit1 construct; scr, MCF-7 cells stably transfected with a scrambled short hairpin RNA (shRNA) control construct; ‘—’, both primary antibodies omitted. All images are representatives of at least three independent experiments. Lysate controls are presented in the lower panels. *Heavy chain of precipitating antibody.