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. 2016 Jan 5;2:15039. doi: 10.1038/celldisc.2015.39

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Copyright © 2016 SIBS, CAS

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HNit1 directly binds to the Armadillo-repeat region in β-catenin. In vitro pull-down assay were performed with purified recombinant GST-β-catenin and MBP-hNit1 fusion proteins as indicated. GST and MBP were used as a control. Deletion constructs of β-catenin (GST-β-cat (aa1–781); GST-β-cat-N-term (aa1–119); GST-β-cat-core (aa120–683) and GST-β-cat-C-term (aa683–781)) were used to map the hNit1-binding site in β-catenin. Protein complexes were isolated with glutathione (GSH)-agarose beads and analysed by western blotting with a monoclonal anti-MBP or a polyclonal anti-GST antibody. The image is a representative of at least three independent experiments.