Figure 6.
The Rosetta-Stone fusion protein dNitFhit binds to Armadillo and β-catenin, and represses Wg-mediated transcription. (a) FLAG3-Armadillo and dNitFhit-myc6 form a complex in co-immunoprecipitations from lysates of transiently transfected HEK-293 cells. Similar complex formation was observed for the dNit and dFhit domain expressed alone. Protein complexes were analysed by western blotting with anti-myc (9E10) antibody. Lysate controls are shown in the lower panels. *Light chains, **heavy chains of the precipitating antibody. (b) Engrailed-Gal4-driven expression of UAS-dNitFhit, UAS-dNit, UAS-dFhit or combined UAS-dNit+UAS-dFhit repress Senseless expression in Drosophila wing imaginal discs. The repressive effect is manifested as reduced intensity and width/thickness of immunostained Senseless pattern. Coexpression of UAS-GFP was used to mark the posterior part of the wing discs where the Engrailed-Gal4 driver is active. Transgenic fly line expressing empty vector was used as a wild-type control. Analysis of Senseless expression at 29 °C is enhancing the effect as shown in the lower panels.