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. 2016 May 9;7:80. doi: 10.3389/fgene.2016.00080

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Copyright © 2016 Rahmani, Zimmermann, Grill, Kennedy, Oberg, White, Poland and McKinney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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WGCNA cluster sizes for flu vaccine RNA-Seq co-expression network. Each of the 20 clusters (from 4965 transcripts) is labeled by its most enriched immune system pathway (of the left) and by its hub gene (gene with highest within module degree) on the right. These clusters contain 2543 of the original 4965 because 2422 fell into the “unassigned” cluster. Algorithm parameters and hard threshold were swept with the goal of finding a module size distribution without extremely small or large modules. Parentheses in pathway names indicate source of annotation (Reactome, KEGG, NCI-PID, and BioCarta).