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. 2016 May 9;7:170. doi: 10.3389/fphys.2016.00170

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Copyright © 2016 Gao, Wang, Li, Zhang, Ye and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of differential expression by quantitative real-time polymerase chain reaction (qRT-PCR). (A) Eight genes associated with the ET biosynthetic/metabolic process or signaling pathway. (B) Nine genes related to UDP-glucuronosyl/UDP-glucosyltransferase activity, carbohydrate transport, and metabolism or signal transduction mechanisms. (C) Six genes related to the biosynthetic or metabolic process of phenylpropanoids. (D) Three genes responded to JA biosynthesis. (E) Comparison between the gene expression ratios obtained from RNA-Seq data (y-axis; log₂) and qRT-PCR (x-axis; log₂).