Fig. 4.

In situ hybridization of rbcL mRNA (A–G) and in situ immunolocalization of Rubisco rbcL (H–K) and PEPC (L–O) with longitudinal leaf sections from base to tip of Suaeda aralocaspica at four stages of development: Stage 1 (D, H, L), Stage 2 (E, I, M), Stage 3 (F, J, N) and Stage 4 (G, K, O). The dark purple signal indicates the specific hybridization to an antisense mRNA probe for rbcL mRNA (A, B, D–G). Yellow particles indicate peptide antibody labeling (H–O). (A, B) Basipetal gradient of rbcL transcript accumulation from base (left) to tip (right). (C) Sense probe control showing the very low background staining that occurs in mRNA sense strand hybridization reactions. E, epidermis; C, chlorenchyma; H, hypoderm; WS, water storage. Scale bars: 200 μm for A, 100 μm for B; 10 μm for C–O.