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. 2016 Mar 8;67(9):2655–2664. doi: 10.1093/jxb/erw096

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — VIGS of SLTAB2 DMR1. (A) At 3 weeks post-infection, plants infected with TRV-DMR1b displayed large chlorotic sectors. Between 13 and 16 plants were infected for each condition. (B) These sulfurea-like sectors remained in later leaves at 2 months after infection. (C) Methylation of DMR1 is detectable in chlorotic sectors. The proportion of methylation is calculated from the ratio of amplicons in the McrBC-digested versus undigested samples (as determined by qPCR). If all alleles are highly methylated (100% methylation), they will be digested by McrBC and no amplification will occur during qPCR.