Table 1.
Cross-validation of proteins identified by iTRAQ- and 2D gel-based proteomic analyses
| Gene locus | Protein description | emPAI ratio* | SC_unNor ratio* | Spots | iTRAQ ratio* |
|---|---|---|---|---|---|
| AT1G79040.1 | Photosystem II subunit R | 0.23 | 0.5 | 529 | 0.55 |
| AT2G33800.1 | Ribosomal protein S5 | 0.05 | 0.03 | 1508/1507 | 0.56 |
| AT1G64510.1 | Ribosomal protein S6 | 0.57 | 0.8 | 1216 | 0.6 |
| AT4G32260.1 | ATPase subunit b | 0.55 | 0.7 | 1216 | 0.61 |
| AT3G52150.1 | Plastid-specific ribosomal protein 2 | 0.63 | 0.7 | 1204 | 0.66 |
| AT1G67090.1 | Ribulose bisphosphate carboxylase small chain 1A | 0.41 | 0.7 | 969 | 0.68 |
| AT5G07020.1 | Proline-rich protein family | 0.14 | 0.3 | 969 | 0.73 |
| AT1G11860.1 | Glycine cleavage T-protein | 2.5 | 1.6 | 1375 | 1.33 |
| AT5G26000.1 | Myrosinase 1 | 4.2 | 4 | 239/231 | 1.38 |
| AT2G39730.1 | Rubisco activase | 2.2 | 1.4 | 1400 | 1.48 |
| AT1G23310.1 | Glutamate:glyoxylate aminotransferase | 2.2 | 1.8 | 477 | 1.54 |
| AT5G43940.1 | Alcohol dehydrogenase class-3 | 1.34 | 2.5 | 554 | 1.4 |
| AT4G26530.1 | Fructose-bisphosphate aldolase 5 | 2.6 | 1.9 | 231 | 1.66 |
| AT3G60750.1 | Transketolase | 2.7 | 1.6 | 231 | 1.82 |
emPAI ratio, protein ratio calculated by Exponentially Modified Protein Abundance Index; SC_unNor ratio, protein ratio calculated by Spectrum Counting_unNormalization. * The protein ratio is rps5:wild-type.