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. 2016 Mar 29;67(9):2745–2760. doi: 10.1093/jxb/erw107

Fig. 2.

Fig. 2.

Dominant negative mutants are more sensitive to submergence than Col-0. (A) The expression of SnRK1.1 in Col-0, SnRK1.1 K48M, SnRK1.1 T175A, and SnRK1.1 T175D quantified by qRT-PCR. TUBULIN mRNA was the internal control. (B) The phenotype of 4-week-old Col-0 and dominant negative mutants (SnRK1.1 K48M, SnRK1.1 T175A, and SnRK1.1 T175D), after submergence for 60h in the dark at 22 °C and recovery for 4 d, bar =1cm. (C) After submergence for 60h and recovery for 4 d, the percentage of necrotic leaf area was used as the damage index. The data represent means ±SE from six independent biological replicates. Data from the lines with the same lowercase letters were significantly different from Col-0, which was determined by Student’s t test (P <0.05). (D) The change in the AMP/ATP ratio in 9-d-old seedlings of Col-0 and SnRK1.1 mutants at different stages of submergence at room temperature in the dark. (E) The relative fold change of ATP and AMP in 9-d-old seedlings of Col-0 and SnRK1.1 mutants under submergence. The data represent means ±SD from four independent biological replicates. Statistical differences between submerged and control samples were determined by Student’s t test. *, P <0.05.