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. 2016 May 7;67(9):2829–2845. doi: 10.1093/jxb/erw122

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 9. — Yeast one hybrid assay of interaction of VaNAC26 on NACRS and its mutation sequence. Yeast transformants were examined by growth performance on SD/-Leu/-Ura with 500mg L⁻¹ of AbA. The pAbAi-P53-transformed Y1HGold was set as negative control and the pGADT7-P53 plus pAbAi-P53 transformants were set as positive control. (A) Structural illustration of pGADT7-VaNAC26 and pAbAi-bait vectors. (B) and (C) represent the co-transformed yeast cells growth conditions on SD/-Leu/-Ura media and SD/-Leu/-Ura with 500mg L⁻¹ of AbA, respectively. P, positive control; N, negative control; 1, transformants with pGADT7-VaNAC26 and pAbAi-4×NACRS; 2, transformants with pGADT7-VaNAC26 and pAbAi-4×mutants.