Table 3.

Characteristics of Oryza sativa L. Japonica cultivar Taichung wild-type and PEPCK transgenic lines (TRIM line M0035095) (Hsing et al., 2007)

Relative expression of PEPCK compared to glutaredoxin and PEPCK enzyme activity were measured in leaves. Copy number was determined to be one for the inserted gene. Guttation fluid was pooled from leaves of wild-type, heterozygous and homozygous plants, the volume measured and amino acid contents determined for each genotype. Values are the mean of three measurements from six plants ±SE. See Supplementary Tables S5–S7.

Plant	Gene expression mean ±SE (% wild type)	Enzyme activity mean ±SE (µmol min −1 g −1 FWt) (% wild type)	Total amino acid content (nmol)	Guttation fluid volume (µl)
Wild type	1.98±0.05 (100)	0.062±0.005 (100)	9.70±0.85	33±3
Heterozygote	3.01±0.22 (152)	0.091±0.006 (147)	4.92±0.46	27±3
Homozygote	5.71±0.42 (288)	0.135±0.019 (218)	2.51±0.13	13±1