Fig. 1.

NS398 and Thiostrepton inhibits cell viability in CRC cell lines (a) HT29, DLD1, Caco-2, HCT-15 and LOVO cells were lysed and immuno-blotted with Cox-2, FoxM1 and Beta-actin antibodies (b) Caco-2 and HT29 cell lines were treated with 50 and 100 μM NS398 for 48 h. Proteins were lysed and immunoblotted with antibodies against FoxM1, Cox-2, p-AKT, total AKT and Beta-actin. c HT29 cells were either transfected with 50 and 100nM siRNA, specific against Cox-2 or scrambled siRNA for 48 h. Cells were lysed and equal amounts of proteins were immuno-blotted with antibodies against FoxM1, Cox-2, p-AKT, total AKT and Beta-actin. d Caco-2 and HT29 cell lines were treated with 5 and 10 μM Thiostrepton for 48 h. Proteins were lysed and immunoblotted with antibodies against FoxM1, Cox-2, p-AKT, total AKT and Beta-actin. e HT29 cells were either transfected with 50 and 100nM siRNA, specific against FoxM1 or scrambled siRNA for 48 h. Cells were lysed and equal amounts of proteins were immuno-blotted with antibodies against FoxM1, Cox-2, p-AKT, total AKT and beta-actin. f Caco-2 and HT29 cell lines were incubated with 0-100 μM NS398 for 48 h. Cell viability was measured by MTT assays as described in Materials and Methods. The graph displays the mean +/- SD (standard deviation) of three independent experiments, * p < 0.05, statistically significant (Students t-test). g Caco-2 and HT29 cell lines were incubated with 0-25 μM Thiostrepton for 48 h. Cell viability was measured by MTT assays as described in Materials and Methods. The graph displays the mean +/- SD (standard deviation) of three independent experiments, * p < 0.05, statistically significant (Students t-test)