Fig. 3.

Combination of NS398 and Thiostrepton at sub-optimal doses inhibits cell viability and decreases cell invasion/migration capability of CRC cells. a Caco-2 and HT29 cell lines were incubated with either 10 μM NS398 or 5 μM Thiostrepton alone or in combination for 48 h. Cell viability was measured by MTT assays as described in Materials and Methods. The graph displays the mean +/- SD (standard deviation) of three independent experiments, * p < 0.05, statistically significant (Students t-test). b Colony formation assays were performed as described in Materials and Methods. HT29 cells were treated with either 10 μM NS398 or 5 μM Thiostrepton alone or in combination for 48 h. Subsequently, cells were plated on Soft agar plates for 4 weeks. After 4 weeks, plates were stained and manually counted. Bar graph denotes number of colonies counted manually. c HT29 cells were treated with 10 μM NS398 and 5 μM Thiostrepton alone or in combination for 48 h. Following treatment, Invasion-Migration assay were performed as described in the material and methods section. d Caco-2 and HT29 cell lines were treated with 10 μM NS398 and 5 μM Thiostrepton either alone or in combination for 48 h. Proteins were lysed and immunoblotted with antibodies against FoxM1, Cox-2, p-AKT, total AKT, MMP-9 and Beta-actin