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. 2015 Jul 10;14:131. doi: 10.1186/s12943-015-0406-1

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© Ahmed et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Combination of NS398 and Thiostrepton at sub-optimal inhibits growth of HT29 xenograft and down-regulates expression of Cox-2, FoxM1 and p-AKT. Nude mice at 6 weeks of age were injected with 10 million HT29 cells. a The volume of each tumor was measured every week. The average (n = 6) tumor volumes in vehicle-treated mice () and mice treated with indicated doses of NS398 (15 mg/kg ), Thiostrepton (150 mg/kg ) and a combination of NS398 and Thiostrepton (NS398 15 mg/kg + Thiostrepton 150 mg/kg; ) were plotted. The results are expressed as mean ± SD (n = 6). *P <0.001 compared with vehicle-treated mice. b After 5 weeks of treatment, mice were sacrificed and tumor weights were measured. The results are expressed as mean ± SD. *P < 0.001 compared with vehicle-treated mice by Student t test. c Representative tumor images of vehicle-treated mice and mice treated with NS398, Thiostrepton and combination of both agents after necropsy. d Whole cell homogenates from mice treated with vehicle, 15 mg/kg NS398, 150 mg/kg Thiostrepton and a combination of 15 mg/kg NS398, + 150 mg/kg Thiostrepton were immuno -blotted with FoxM1, Cox-2, p-AKT, total AKT, MMP-9, caspase-3, and Beta-actin antibodies

Inline graphic — Combination of NS398 and Thiostrepton at sub-optimal inhibits growth of HT29 xenograft and down-regulates expression of Cox-2, FoxM1 and p-AKT. Nude mice at 6 weeks of age were injected with 10 million HT29 cells. a The volume of each tumor was measured every week. The average (n = 6) tumor volumes in vehicle-treated mice () and mice treated with indicated doses of NS398 (15 mg/kg ), Thiostrepton (150 mg/kg ) and a combination of NS398 and Thiostrepton (NS398 15 mg/kg + Thiostrepton 150 mg/kg; ) were plotted. The results are expressed as mean ± SD (n = 6). *P <0.001 compared with vehicle-treated mice. b After 5 weeks of treatment, mice were sacrificed and tumor weights were measured. The results are expressed as mean ± SD. *P < 0.001 compared with vehicle-treated mice by Student t test. c Representative tumor images of vehicle-treated mice and mice treated with NS398, Thiostrepton and combination of both agents after necropsy. d Whole cell homogenates from mice treated with vehicle, 15 mg/kg NS398, 150 mg/kg Thiostrepton and a combination of 15 mg/kg NS398, + 150 mg/kg Thiostrepton were immuno -blotted with FoxM1, Cox-2, p-AKT, total AKT, MMP-9, caspase-3, and Beta-actin antibodies