Fig. 7.

Transcription kinetics of SfMNPV ColA ORFs 023 and 024. Spodoptera frugiperda larvae were exposed to SfMNPV ColA and whole RNAs were isolated from sacrificed animals at different intervals post-infection. Then, cDNA with polyT primer was generated for each sample and PCR assays were done using specific primers which amplify fragments of different ORFs from SfMNPV ColA genome [polyhedrin (polh), immediate-early 0 (ie-0), ORF023 and ORF024]. Figure shows a photo cut-out showing the amplification bands resolved by agarose gel electrophoresis. The SfMNPV ColA genome was used as positive control and water was used as negative control