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. 2016 Jan 18;7:14. doi: 10.1186/s13287-015-0261-4

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© Yaochite et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — T1D-MSCs efficiently inhibit in vitro T-cell proliferation. In coculture assays, different concentrations of phytohemagglutinin (PHA)-stimulated allogeneic peripheral blood mononuclear cells (PBMCs) labeled with CFSE were cultured in the presence of C-MSCs (n = 6) or T1D-MSCs (n = 3). The percentage of CD3⁺ T-lymphocyte proliferation was determined by flow cytometry. a Percentages of lymphocyte proliferation. b Percentages of lymphocyte proliferation inhibition. Data expressed as mean ± SD. C-MSCs mesenchymal stromal cells from bone marrow of healthy individuals, MSC multipotent mesenchymal stromal cell, T1D-MSCs mesenchymal stromal cells from bone marrow of newly diagnosed T1D patients