FIG. 2.

Maternal HF diet intake resulted in increased primordial follicle assembly and reduced AMH in offspring ovaries at P4. Representative photomicrographs of hematoxylin and eosin stained P4 offspring ovaries from dams fed CON (a) and HF (b). Maternal HF diet during pregnancy and lactation resulted in increases of 67% and 69%, respectively, in the number of primordial (c) and transitioning (d) follicles (n = 8, CON; n = 8, HF). Representative photomicrographs of AMH (e, f, g) and AMHRII (i, j, k) immunostaining of P4 offspring ovaries from CON (e, i, respectively) and HF (f, j, respectively) dams with negative controls (g, k, respectively). Maternal HF diet during pregnancy and lactation resulted in a significant decrease in positive AMH and AMHRII immunostaining, expressed as a proportion of immunostaining per total area analyzed (h, l, respectively) (n = 5, CON; n = 4, HF). Maternal HF diet during pregnancy and lactation resulted in a significant increase in activated caspase-3 immunostaining, expressed as a proportion of positive immunostaining per total area analyzed (m) (n = 3, CON; n = 3, HF). Data are mean ± SEM fold changes relative to CON offspring for follicle counts. Student unpaired t-test, *P < 0.05; **P < 0.01. Bars = 100 μm (a–c, e–g, and i–k).