Fig. 1.

RT-PCR of SAA transcripts in human bone: RNA was isolated from different bone preparations, reverse transcribed, and cDNAs of SAA1, SAA2, and SAA4 amplified using specific forward and reverse oligonucleotide primers (Supplement, Table I). The PCR products were separated on 1.5% agarose gels. T, trabecular bone; C, cortical bone; BM, bone marrow; P, positive control, HUH-7 cells; N1, negative control—RNA template: negative controls were done for all samples; N2, negative control—water template. To ensure that equal amount of cDNA was used as a template RT-PCR for GAPDH was made as a control. One representative experiment out of three is shown.