Fig. 3.

RT-PCR of cytokine receptors, SAA and SAF-1 in human osteosarcoma cells: MG-63 cells were stimulated with different cytokines (10 ng/ml) for 24 h. A: Cytokine receptor transcripts as well as (B) SAA and SAF-1 transcripts were amplified using specific oligonucleotide primers (Supplement, Table I). The PCR products were separated on 1.5% agarose gels. NS, non-stimulated; P, positive control: RNA was isolated from HUH-7 cells for SAF-1, SAA, cytokine receptors except IL-1R2, where RNA from THP-1 cells was used; N1, negative control—RNA template: negative controls were done for all samples; N2, negative control—water template. To ensure that equal amount of cDNA was used as a template RT-PCR for GAPDH was made as a control. One representative experiment out of three is shown.