Fig. 5.

Western blot of SAF-1 and SAA4 in osteosarcoma cells: MG-63 cells were stimulated with 10 ng/ml IL-1β up to 6 h in six-well plates. The cells were lysed and the nuclear fraction was isolated as described in Materials and Methods. The protein was subjected to SDS–PAGE and transferred to membranes. Western blot experiments were performed using polyclonal anti-SAF-1 antiserum or sequence-specific anti-human SAA4 peptide antisera as primary antibodies. One representative experiment out of two is shown.