Fig. 6.

RT-PCR of SR-BI/II and FPRL-1/ALX in human MG63 cells: Cells were stimulated with different cytokines (10 ng/ml) for 24 h. Human SR-BI/II and FPRL-1/ALX transcripts were amplified using specific oligonucleotide primers (Supplement, Table I). The PCR products were separated on 1% agarose gels. NS, non-stimulated; P, positive control: RNA was isolated from differentiated THP-1 cells for SR-BI/II, for FPRL-1/ALX, HUH-7 genomic DNA was used; N1, negative control—RNA template: negative controls were done for all samples; N2, negative control—water template. To ensure that equal amount of cDNA was used as a template RT-PCR for GAPDH was made as a control. One representative experiment out of three is shown.