Table 1.

Real-time RT-PCR analysis of liver tissue in ob/ob mice and lean littermates before ischemia and after 40 min of ischemia and 6 h of reperfusion

	Lean Control	Lean I-R	ob/ob Control	ob/ob I-R
Cytokines
TNF-α	1.00±0.07	1.21±0.17	1.00±0.17	0.64±0.09‡
TNFR1	1.00±0.43	0.85±0.17	0.65±0.06	0.53±0.18
IL-1β	1.00±0.18	8.60±1.88*	1.89±0.22†	4.29±0.15*‡
IL-1R	1.00±0.07	2.89±0.37*	0.29±0.01†	0.62±0.19‡
IL-6	1.00±0.07	0.81±0.15	0.82±0.04	0.86±0.12
IFN-γ	1.00±0.32	1.21±0.30	1.61±0.64	3.87±0.72‡
Chemokines
MIP-2	1.00±0.15	4.74±0.83*	1.17±0.21	4.03±0.63*
mKC	1.00±0.40	8.08±1.24*	0.25±0.09	2.83±0.72*‡
Adhesion molecules
ICAM-1	1.00±0.33	1.82±0.20	0.85±0.16	1.17±0.18‡
VCAM-1	1.00±0.07	0.66±0.11	0.94±0.05	0.82±0.07
Others
iNOS	1.00±0.29	1.04±0.19	0.95±0.39	1.24±0.36
COX-2	1.00±0.13	0.60±0.09*	0.94±0.06	0.82±0.06
TSP-1	1.00±0.07	6.35±0.96*	1.96±0.49	7.99±1.69*
HO-1	1.00±0.20	6.22±0.70*	1.20±0.05	1.65±0.33‡
UCP-2	1.00±0.20	0.64±0.09*	3.21±0.28†	1.55±0.06*
EGR-1	1.00±0.28	10.38±1.80*	1.64±0.52	12.42±2.36*
SOD-1	1.00±0.12	0.55±0.16	1.12±0.03	0.79±0.08*

The expression level of each gene was first normalized to that of β-actin in each individual sample, and then the relative differences between groups were expressed as relative increases or decreases setting the lean control group as 1.00. Values are presented as means ± SE of 3–5 animals per group. TNF-α, tumor necrosis factor-α; TNFR1, TNF receptor type 1; IL-1β, interleukin 1β; IL-1R, IL-1 receptor; IL-6, interleukin 6; IFN-γ, interferon γ; MIP-2, macrophage inflammatory protein-2; mKC, murine keratinocyte-derived chemokine; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase 2; TSP-1, thrombospondin 1; HO-1, heme oxygenase-1; UCP-2, uncoupling protein-2; EGR-1, early growth response factor-1; SOD-1, superoxide dismutase-1.

^*P < 0.05 vs. respective control groups

^†P < 0.05 vs. lean control group

^‡P < 0.05 vs. lean ischemia-reperfusion (I-R) group.