FIGURE 7. Increased number of apoptotic particles in the phagosomal compartment in macrophages that ingested SM4s-painted apoptotic C26.
Murine peritoneal macrophages were grown on cover slips placed in 6-well plates and cocultured with apoptotic cells at 37°C for 1 h and subjected to ultrastructural analysis by electron microscopy. A, Quantification of apoptotic particles within phagosomes (arrows) between the two groups. Dense apoptotic material in the phagolysosomes (stars) of apoptotic cell-SM4s phagocytosing macrophages in comparison to the control. B and C, Number of apoptotic particle-containing phagosomes per macrophage and percent of macrophages that contain apoptotic material were evaluated. Results are presented as mean ± SEM from total of 80 macrophages from three experiments. Statistical differences were evaluated by Mann-Whitney U test. ***, p < 0.001.